IOP Conference Series: Earth and Environmental Science, vol: 584,1 (2020)
Quantitative fucoxanthin extract of tropical Padina sp. And Sargassum sp. (Ocrophyta) and its’ radical scavenging activity
Nisa A.A., Sedjati S., Yudiati E.
Abstract
Fucoxanthin is a potential compound in medicinal and nutritional ingredients due to its antioxicant acivity. This study was carried out to determine the fucoxanthin contents and antioxidant activitiy of crude fucoxanthin extract from Padina sp. and Sargassum sp. The crude fucoxanthin was partitioned using some solvents ie. methanol-water and followed by using n-hexane. The total fucoxanthin contents were analysed using High Performance Liquid Chromatography (HPLC) instrument, quantitatively and qualitatively. The total of fucoxanthin contents in Padina sp. was relatively high ( 2.44 mg/g) and followed by Sargassum sp (1.95 mg/g) wet weight, respectively. The antioxidant activity was done spectropohotometrically using diphenylpicrylhidrazyl (DPPH) method. The crude fucoxanthin extract from Padina sp. was showing a moderate antioxidant activity (209.93 ppm), while Sargassum sp was 639.22 ppm. Padina sp. from tropical waters is rich in fucoxanthin. Concerning the unstable fucoxanthin compound, next methods to stabilize the fucoxanthin extract need to explore. © Published under licence by IOP Publishing Ltd.
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