Customize Consent Preferences

We use cookies to help you navigate efficiently and perform certain functions. You will find detailed information about all cookies under each consent category below.

The cookies that are categorized as "Necessary" are stored on your browser as they are essential for enabling the basic functionalities of the site. ... 

Always Active

Necessary cookies are required to enable the basic features of this site, such as providing secure log-in or adjusting your consent preferences. These cookies do not store any personally identifiable data.

No cookies to display.

Functional cookies help perform certain functionalities like sharing the content of the website on social media platforms, collecting feedback, and other third-party features.

No cookies to display.

Analytical cookies are used to understand how visitors interact with the website. These cookies help provide information on metrics such as the number of visitors, bounce rate, traffic source, etc.

No cookies to display.

Performance cookies are used to understand and analyze the key performance indexes of the website which helps in delivering a better user experience for the visitors.

No cookies to display.

Advertisement cookies are used to provide visitors with customized advertisements based on the pages you visited previously and to analyze the effectiveness of the ad campaigns.

No cookies to display.

Philippine Journal of Science, vol: 147,1 (2018)

Preliminary investigation of the carotenoid Composition of Erythrobacter sp. Strain KJ5 by high-performance liquid chromatography and mass spectrometry

Juliadiningtyas A.D., Pringgenies D., Heriyanto, Salim K.P., Radjasa O.K., Shioi Y., Limantara L., Brotosudarmo T.H.P.

Abstract

Separation and identification of carotenoids (Cars) from aerobic marine bacterium Erythrobacter sp. strain KJ5 are reported. The cells of Erythrobacter sp. were grown in a Shioi medium at 28.5°C for three days. Among the four solvents tested, the mixture of methanol and acetone (3:7, v/v) was determined as the optimum solvent for Car extraction from the cells by measuring its absorption spectrum. The Cars were separated via reversed-phase high-performance liquid chromatography using a C8 column and identified by a UV-Vis photodiode array detector and an electrospray ionization mass spectrometry. Bacteriochlorophyll a was not detected from the extracts of cells grown under both light and dark conditions. At least 16 peaks of Cars were separated, wherein eleven peaks showed the same absorption spectrum with ?max at 452-453 nm and at 478-480 nm. The other five peaks had an additional absorption peak at 340 nm, which belongs to cis-isomeric form. Two peaks of Cars were identified to be zeaxanthin and ?-carotene. © 2018, Department of Science and Technology. All rights reserved.

Keyword: Carotenoids; Erythrobacter sp; High-performance liquid chromatography; Mass spectrometry; Zeaxanthin; ?-carotene

DOI

× How can I help you?