Asian Journal of Applied Sciences, vol: 9,2 (2016)
Identification of bacteria indigenous selected Rawa Pening lake owns best degradation capability of the organophosphate pesticides (malathion and profenofos)
Isworo S., Purwanto, Sabdono A.
Abstract
Background and Objectives: The process of selection and identification is a very important thing to get the names and types of bacteria that have the best bioremediation capabilities so that the bioremediation process can run optimally. Identification of selected bacteria which has the best degradation ability on (malathion and profenofos) is one way to get the ultimate bioremediation agent. Methodology: This study successfully selecting three best bacteria based on their ability to degradation of the malathion and profenofos perfectly is a bacterium with a code RPL-1, RPL-5 and TRA-5. The selected bacteria are then identified by molecular biology. Results: Results Identification of molecular genetics and phylogenetic analysis of bacteria that the level of equality test with the maximum bacterial identification RPL-1 is approximately 87%, with the results of phylogenetic tree analysis has the closest kinship relations as a bacterium with the name Oceanobacillus iheyensis. While the results of the analysis of the level of similarity with the bacterial maximum identity RPL-5 is approximately 99% with the results of phylogenetic tree analysis test showed the closest kinship with bacteria Exiguobacterium profundum. Conclusion: The results of the analysis of the maximum levels of bacterial identity TRA-5 is equal to 98% with the results of phylogenetic tree analysis test against bacterial isolates TRA-5 has addressed a very close kinship with Bacillus firmus. Bacteria have been registered in the DNA Data Bank of Japan, DDBJ Center, National Institute of Genetics, Shizuoka, Japan. © 2016 Slamet Isworo et al.
Keyword: Bioremediation; Malathion; Molecular biology degradation; Pollution of pesticide; Profenofos