IOP Conference Series: Earth and Environmental Science, vol: 246,1 (2019)
Analysis of ?-cryptoxanthin from yellow pigmented marine bacterium Erythrobacter sp. kj5
Setiyono E., Brotosudarmo T.H.P., Pringgenies D., Heriyanto, Prihastyanti M.N.U., Shioi Y.
Abstract
The objectives of this study were to isolate and analyze ?-cryptoxanthin from yellow pigmented marine bacterium Erythrobacter sp. KJ5. The pigments from Erythrobacter sp. KJ5 were extracted from cells with methanol:acetone (7:3, v/v). ?-cryptoxanthin standard was isolated from citrus fruit peel using 100% acetone and purified by high-performance liquid chromatography (HPLC) using a C30 column. The existence of ?-cryptoxanthin in Erythrobacter sp. KJ5 was determined based on spectral properties and co-chromatography analyses after adding the standard ?-cryptoxanthin. The co-chromatography results showed that peak 5 at retention time 33.32 min sharply spiked without changing maximum wavelengths (?max)at 453 and 480 nm after adding the standard ?-cryptoxanthin. These results likely conclude that peak 5 was ?-cryptoxanthin. © 2019 IOP Publishing Ltd.
Keyword: Carotenoid; Co-chromatography; Erythrobacter sp. KJ5; HPLC; ?-cryptoxanthin