AACL Bioflux, vol: 14,3 (2021)
Chlorpyrifos pesticide-degrading bacteria isolated from bivalves of surabaya coastal waters, east java, indonesia
Suryono C.A., Subagiyo, Sabdono A.
Abstract
Waste from inhabited regions, factories and maricultural practices enter coastal waters through movement and penetration. A study was undertaken to determine the diversity of chlorpyrifos-degrading bacteria isolated from bivalves of Surabaya coastal waters, Indonesia. 14 of 127 bacterial strains isolated from 18 bivalves were selected due to their capability of degrading chlorpyrifos herbicide. These isolates were different in the ability of chlorpyrifos degradation. They utilize chlorpyrifos as the carbon and energy for growth. Their initial degradation at 50 mg L?1 concentration within the first 4 days ranged between 7.43 and 68.14%. The 16S rRNA gene sequence analyses indicated that the majority of the isolates belonged to Vibrio genera (10 strains), followed by Virgibacillus spp. (three strains) and Bacillus (1 strain). Virgibacillus salarius strain KS4.3 was selected for further study on kinetic growth and chlorpyrifos utilization. These bacterial strains have a great potential utility for the bioremediation of Surabaya coastal waters and for decreasing levels of toxic chlorpyrifos pesticide residues in bivalves. © 2021, BIOFLUX SRL. All rights reserved.
Keyword: Bacillus; Biodegradation; Herbicide; Java Sea; Vibrio; Virgibacillus