IOP Conference Series: Earth and Environmental Science, vol: 750,1 (2021)
Preliminary Screening of Carbohydrase-Producing Bacteria from Chaetomorpha sp. In Sepanjang Beach, Yogyakarta, Indonesia
Wijaya A.P., Sibero M.T., Zilda D.S., Windiyana A.N., Wijayanto A., Frederick E.H., Murwani R., Wijayanti D.P., Sabdono A., Pringgenies D., Radjasa O.K.
Abstract
Enzyme is a biocatalyst that has been known for its function in various industrial applications. One of the potential natural producers of enzymes is seaweed associated bacteria. Seaweed associated bacteria has been studied as a natural source of carbohydrase such as carrageenase, alginate lyase, and agarase. The purpose of this study was to determine the potential of seaweed associated bacteria from Gunung Kidul, Yogyakarta, Indonesia as a source of carbohydrase enzymes. A total of 13 bacterial isolates were successfully isolated from Chaetomorpha sp. in Sepanjang Beach. Enzymatic activity was measured through cultivation of each bacterium on semi-solid media with addition of substrate of each enzyme. The results showed that 3 isolates (GK.6.10; GK.6.11; GK.6.12) had clear zones around the growing colonies in medium containing 0,2% starch and 2% ?-carrageenan. Meanwhile, 4 isolates (GK.6.3; GK.6.10; GK.6.11; and GK.6.12) showed clear zones in medium containing 0,5% alginate and 2% agar indicating the production of alginate lyase and agarase enzyme. Bacteria GK.6.10; GK.6.11; and GK.6.12 were identified as Salinicola zeshunii, Bacillus piscis, and Bacillus licheniformis with BLAST homology 95.23%, 99.46%, and 99.26%. © Published under licence by IOP Publishing Ltd.
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