AACL Bioflux, vol: 12,5 (2019)
Anti MDR acinetobacter baumannii of the sponges-associated fungi from Karimunjawa national park
Sabdaningsih A., Cristianawati O., Sibero M.T., Aini M., Radjasa O.K., Sabdono A., Trianto A.
Abstract
Marine environment is an enormous source of bioactive compounds with potential as a pharmaceutical agents. Such organisms that produce potential bioactive compounds are sponges, which are filter feeders with many associated microorganisms. Sponges-associated fungi have been reported as an antibacterial sources. This study aimed to obtain and to identify the potential sponges-associated fungi that are active against mulltidrug resistance (MDR) Acinetobacter baumannii. The sponges were collected from Karimunjawa Islands. The MDR A. baumannii was obtained from Kariadi hospital that is resistant against at least 11 antibiotics. The fungal symbionts were isolated by tapping method followed by colony isolation based on the morphological characteristics. Antibacterial assay was conducted by disk diffusion agar. The potential fungal isolates were cultured on malt extract broth (MEB). The potential isolate was identified based on the molecular analysis. The crude extract of KJ-S03.1 was characterized with Thin Layer Chromatography and phytochemical test. A total of 24 fungi were isolated from ten sponges. Among the isolates, the fungus KJ-S03.1 showed the most potential based on the activity against MDR A. baumannii. The molecular analysis identified that the fungus KJ-S03.1 is Aspergillus nomius. The sequence was deposited into Genbank with accession number LC415575.1. The extract of the isolate contains alkaloid, quinone and flavonoid. Isolate A. nomius KJ-S03.1 is a potential source of antibacterial agent that is active against the MDR A. baumannii. © 2019, BIOFLUX SRL. All rights reserved.
Keyword: Acinetobacter baumannii; Aspergiillus nomius; Karimunjawa; Marine fungi; MDR; Sponges