Journal of Physics: Conference Series, vol: 884,1 (2017)
Effect of centrifugation at 7,000 g, 8,000 g, and 9,000 g on the salivary protein profile ?30 kDa
Baskoro B.D., Nugraha R.A., Puspitawati R., Redjeki S.
Abstract
There are no established standard operational procedures of centrifugation for protein separation. Centrifugation at 10,000 g separates ?30 kDa salivary proteins. The aim of this study is to determine the effect of centrifugation at 7,000 g, 8,000 g, and 9,000 g on the salivary protein profile and the frequency of ?30 kDa salivary proteins. Post-centrifuged salivary supernatant was analyzed using SDS-PAGE. An increased centrifugation speed resulted in the decreased frequency of ?30 kDa salivary protein emergence. Each centrifugation speed resulted in a different protein profile. Centrifugation at 7,000 g, 8,000 g, and 9,000 g influences the frequency of salivary protein emergence and protein profiles ?30 kDa. © Published under licence by IOP Publishing Ltd.
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