AACL Bioflux, vol: 13,4 (2020)
Characteristics of bioactive compounds of Holothuria atra (Jaeger, 1833) associated bacteria
Santosa G.W., Djunaedi A., Susanto A.B., Pringgenies D., Ariyanto D.
Abstract
Sea cucumbers have bioactive compounds with health benefits. These active compounds can be used as raw materials for marine aquaculture. This study aims to determine the potential of sea cucumber intestine-associated bacteria as a raw material for aquaculture through the investigation of its characteristics using the GC-MS method. Holothuria atra samples were collected from December 2018 to April 2019, from Bandengan, Jepara waters, Central Java, Indonesia. The solid-liquid method is used in the extraction process. Open Column Chromatography (OCC) is used in the fractionation process. Anti-bacterial sensitivity test was carried out using the Kirby-Bauer agar diffusion method. This study found 26 bacterial isolates resulting from the isolation of black sea cucumber (H. atra) symbionts. Of the 26 bacterial isolates, 9 bacterial isolates were found to have antibacterial activity against multi-drug resistant bacteria. 2 isolates were selected from the 9 test results, namely: T.1.2, which formed a 7.3 mm zone of inhibition against Escherichia coli and 7.8 mm against Staphylococcus aureus; and T.1.13, which was only active against S. aureus with an inhibition zone of 8.2 mm. Identification of selected isolates showed that isolate T.1.2 matched with Bacillus manliponensis species with 97% homology and that isolate T.4.1.25 matched with Bacillus oceanisediminis, with 95% homology. GC-MS analysis found methyl hexadecanoate/methyl palmitate, bis (2-ethylhexyl)-1,2-benzene dicarboxylate, 9-octadecenal, glycerol-1,3-dihexadecanoate and diisooctyl-1,2-benzene dicarboxylate in sea cucumber intestine-associated bacteria. © 2020, BIOFLUX SRL. All rights reserved.
Keyword: Culture; GCMS; Holothuroidea; Intestines