IOP Conference Series: Earth and Environmental Science, vol: 246,1 (2019)
Potential and Enzymatic Characterizations of Marine Yeast for Pufas from Balai Taman Nasional Karimunjawa
Devi S.A., Wulandary D.A., Saputra E., Muchlissin S.I., Setyati W.A.
Abstract
Potential of huge mangrove ecosystem in Indonesia, especially in Balai Taman Nasional Karimunjawa, for exploration of potential microorganism. One of them were yeast. PUFA is beneficial as a source of food for low and high-level organisms. The aim of this study is to explored the potential of yeast by enzymatic methods, differences of culture media and molecular identification in Balai Taman Nasional Karimunjawa. The research flow included isolation of yeast from several mangrove ecosystem located in Karimunjawa by purposive sampling methods. The pine pollen used to bait, identification colony morphology, Protease, amylolytic, cellulolytic, Lipase Enzymatic Test, mass culture used a variety of different media, molecular identification used 18S rDNA. The results of the studied obtained 4 isolates with the code (T2, KM1, KM2, KM3) which have the potential on the lipase and protease enzyme. Molecular identification used 18S rDNA analysis showed that all isolates were the yeast. Differences in the use of media also produce various yeast biomass. © 2019 IOP Publishing Ltd.
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