AACL Bioflux, vol: 11,3 (2018)
Protein content and free amino acid composition of abalone (Haliotis asinina) broodstock fed by different fresh macroalgae and formulated diet
Effendy I.J., Hutabarat J., Ambariyanto A., Basuki F.
Abstract
This study aimed to determine the protein content and free amino acid composition of abalone (Haliotis asinina) broodstock fed by fresh macroalgae and formulated diet. Hatchery produced abalones (16 month old) were used in this study. They were fed with 3 species of macroalgae: Gracilaria verrucosa (A), G. edulis (B) and Ulva lactuca (C) and 2 formulated feeds (D and E) for 60 days. The result showed that the protein content of abalone fed by macroalgae and formulated diets from the highest were 18.79±1.19% (B), 17.70±1.05% (A), 17.52±0.26% (C), 16.67±0.31% (D) and 14.59 ±0.56% (E), respectively. All of the fifteen amino acids (L-Isoleucine, L-Glutamic acid, L-Phenylalanine, L-Serine, L-Histidine, L-Threonine, L-Proline, L-Tyrosine, L-Leucine, L-Aspartic acid, L-Lysine HCL, Glycine, L-Arginine, L-Alanine, L-Valine) of broodstock abalone fed formulated feed (D) were higher than those of G. verrucosa, formulated feed (E; with binder agar), G. edulis and U. lactuca. Four free amino acids (Glycine, L-Serine, L-Proline and L-Alanine) content of abalone broodstocks hepatopancreas fed by macroalgae showed no significant different than those fed by formulated feeds. Abalone broodstocks fed by formulated feed (D) and G. verrucosa contained higher glutamic acid, glicyne, aspartic acid and arginine and faster maturation and spawning time compare to those of broodstock fed with formulated feed (e) and natural feed (G. edulis and U. lactuca). © 2018, BIOFLUX SRL. All rights reserved.
Keyword: Abalone; Aquaculture; Broodstocks; Natural and formulated feed