IOP Conference Series: Earth and Environmental Science, vol: 55,1 (2017)
Isolation and Partial Characterization of Bacteria Activity Associated with Gorgonian Euplexaura sp. Against Methicillin-Resistant Staphylococcus aureus (MRSA)
Kristiana R., Ayuningrum D., Asagabaldan M.A., Nuryadi H., Sabdono A., Radjasa O.K., Trianto A.
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) infection has emerged in around the world and has been resistance to ciprofloxacin, erythromycin, clindamycin. The aims of this study were to isolate, to investigate and to characterize bacterial symbionts gorgonian having activity against MRSA. Euplexaura sp. was collected from Panjang Island, Jepara, Indonesia by snorkling 2-5 m in depth. Bacterias were isolated by using spesific media with dilution method. Bacterias were conducted by using the streak method. Antibacterial activity was investigated by overlay method. The potent bacteria was identified by using molecular identification (DNA extraction, electrophoresis, PCR and phylogenetic analysis using 16S rDNA genes with actinobacteria-spesific primers) and bio-chemical test (among 5 isolated bacteria from gorgonian showed activity against MRSA). The strain PG-344 was the best candidat that has an inhibition zone against MRSA. The result of sequencing bacteria is 100% closely related with Virgibacillus salarius. This becomes a potential new bioactive compounds to against MRSA that can be a new drug discovery. © Published under licence by IOP Publishing Ltd.
Keyword: antibacterial; Euplexaura sp.; MRSA; Staphylococcus aureus